Pharmacology experiment - anti-inflammatory activity using carrageenan induced paw oedema methods by Plethysmometer (by software)

Object - To study the anti-inflammatory activity using carrageenan induced paw oedema methods. 

Reference – TripathiK.D: essentials of medical pharmacology Jaypee brothers medical publishers LTD:, 2015.

Requirement- PC & Expharm software 

Theory – Inflammation is a protective response to injury. It occurs in three phases:

(a) The first phase being oedema and swelling with accompanying pain. These effects are produced as a result of the dilation and increased permeability of the blood vessels (veins) due to the release of certain mediators such as histamine, serotonin and kinins etc.

(b) In the second phase, leukocytes migrate to this area and mopping up operations starts.

(c) Second phase is followed by repair, which is ushered in by the proliferation of fibroblasts and synthesis of connective tissue.

The ability of a compound to reduce the local oedema induced in rat paw by various irritants is the most widely used test to screen new non-steroidal anti-inflammatory drugs. Many compounds like formalin, carrageenan, Kaolin, yeast and dextran have been used as irritants to produce oedema.

Plethysmometer is an equipment used to measure paw volume of rat while preforming anti-inflammatory activity.

Pharmacology experiment - anti-inflammatory activity using carrageenan induced paw oedema methods by Plethysmometer (by software)

Procedure – 

• Take 12 animals
• Inject carragenan in paw of animals, wait for 30 min.
• Divide animals into two groups (6 animals in each)
• Administer one group with the drug (Acetyl salicylic acid 300mg/kg) to be tested and other with  vehicle by oral route, wait for 30 min.
• After that evaluate paw volume.
• Record the paw volume change.
• Compare the paw volume change in between vehicle treated and drug treated group.

Observation table –

S.No.	Paw volume in ml
	Vehicle treated rat	Drug treated rat
1
2
3
4
5

Result